Unveiling the Role of β-Glucosidase Genes in Bletilla striata’s Secondary Metabolism: A Genome-Wide Analysis

作者：Mengwei Xu, Hongwei Li, Hongyuan Luo, Jingyi Liu, Kunqian Li, Qingqing Li, Ning Yang, Delin Xu · 发表于：International Journal of Molecular Sciences · 年份：2024 · DOI：10.3390/ijms252313191 · 被引用次数：9 · 研究领域：Biological and pharmacological studies of plants、GABA and Rice Research、Plant tissue culture and regeneration

β-glucosidases (BGLUs) are abundant enzymes in plants that play pivotal roles in cell wall modification, hormone signal transduction, secondary metabolism, defense against herbivores, and volatile compound release. Bletilla striata, a perennial herb revered for its therapeutic properties, lacks a comprehensive analysis of its BGLU gene family despite the critical role these genes play in plant secondary metabolism. This study aims to perform a genome-wide analysis of the BGLU gene family in B. striata (BsBGLU) to elucidate their functions and regulatory mechanisms in secondary metabolite biosynthesis. We conducted a genome-wide screening to identify BsBGLU, followed by phylogenetic analysis to classify these genes into groups. Sequence characteristics were analyzed to predict functional roles. Simple sequence repeat (SSR) markers were examined to assess conservation and polymorphism among different landraces. Expression profiles of BsBGLUs were evaluated under sodium acetate and salicylic acid elicitor treatments and across different tissues. The accumulation of phylogenetic metabolites in different treatments and tissues was also analyzed by HPLC and LCMS detection to explore the correlation between gene expression and metabolite accumulation. A total of 23 BsBGLU genes were identified and classified into eight distinct groups. Sequence analysis suggested diverse functions related to hormone responses, secondary metabolism, and stress resistance. BsBGLUs with SSR sequences w...