Phenomics-assisted genetic dissection and molecular design of drought resistance in rice

作者：Qiaojun Lou, Yunyu Chen, Xin Wang, Yulu Zhang, Tingting Gao, Jiawei Shi, Ming Yan, Fangjun Feng, Kai Xu, Lin Feng, Shangyuan Xie, Xiaoyan Xi, Weikun Li, Yuanyuan Nie, Huan Gao, Hui Xia, Lei Wang, Tiemei Li, Shoujun Chen, Ying Zhu, Jianwei Zhang, Hanwei Mei, Liang Chen, Wanneng Yang, Lijun Luo · 发表于：Plant Communications · 年份：2024 · DOI：10.1016/j.xplc.2024.101218 · 被引用次数：14 · 研究领域：Rice Cultivation and Yield Improvement

Dissecting the drought resistance (DR) mechanism and designing drought-resistant rice varieties are promising strategies to address the challenge of climate change. Here, we selected a typical drought-avoidant (DA) variety IRAT109 and drought-tolerant (DT) variety Hanhui15 as the parents to develop a stable recombinant inbred line (RIL) population (F 8 , 1,262 lines). The de novo assembled genomes of both parents were released. Through re-sequencing of the RIL population, a set of 1,189,216 reliable SNPs were obtained and used for constructing a dense genetic map. Using both aboveground and underground phenomic platforms and multimodal cameras, we captured 139,040 image-based traits (i-traits) of whole plant’s phenotypes in response to drought stress throughout entire rice growth period and identified 32,586 drought-responsive quantitative trait loci (QTLs) including 2,097 unique QTLs. The QTLs related to panicle i-traits occurred on the middle of chromosome 8 over 600 times, while the QTLs related to leaf i-traits on the 5’ end of chromosome 3 over 800 times, indicating potential effect of these QTLs on plant phenotypes. We chose three candidate genes ( OsMADS50, OsGhd8, OsSAUR11 ) related to leaf, panicle, and root traits respectively and verified their functions in resisting drought. Gene OsMADS50 was found to negatively regulate DR by modulating leaf dehydration, grain size, and root downward growth. Furthermore, a total of 18 and 21 composite QTLs significantly related t...