Prevalence and virulence of Vibrio parahaemolyticus isolated from clinical and environmental samples in Huzhou, China

作者：Peng Zhang, Xiaofang Wu, Lei Ji, Yan Wei, Liping Chen, Zhonghao Lu, Deshun Xu, Deshun Xu, Yunfeng Zha, Dafang Xu, Xu D, Fenfen Dong · 发表于：BMC Genomics · 年份：2024 · DOI：10.1186/s12864-024-11106-3 · 被引用次数：13 · 研究领域：Vibrio bacteria research studies、Aquaculture disease management and microbiota、Invertebrate Immune Response Mechanisms

BACKGROUND: Vibrio parahaemolyticus has emerged as the leading cause of seafood-associated infections worldwide. Previous studies have shown that V. parahaemolyticus can be detected in both environmental and clinical samples. However, the molecular characteristics of V. parahaemolyticus isolated from these sources remain unknown. RESULTS: This study examined 128 strains of V. parahaemolyticus isolated from clinical and environmental samples collected between 2020 and 2023 in Huzhou, China. We identified 73 serotypes; O10:K4, O3:K6, and O4:KUT were the dominant serotypes among clinical isolates. We examined the proliferation and motility of major epidemic strains from environmental and clinical samples. Genetic diversity and evolution were assessed by average nucleotide identity (ANI), phylogenetic tree construction, and multilocus sequence typing (MLST). Furthermore, we identified 13 novel sequence types (STs) among the environmental isolates, indicating that V. parahaemolyticus strains are widely distributed and evolve rapidly in the environment in Huzhou, China. We found 206 virulence genes among these isolates, indicating that environmental isolates possess numerous virulence genes. Additionally, we detected 4 strains carrying the tdh or trh gene, which may increase their pathogenicity. The prediction results of antibiotic resistance genes shown that environmental isolates may carry up to 104 resistance genes, compared to 30 in clinical isolates. CONCLUSIONS: We observed t...