RP-HPLC Method Development and Validation for Simultaneous Estimation of Rosuvastatin calcium and Teneligliptin hydrobromide hydrate in Synthetic Mixture

作者：Amitkumar J. Vyas, Harshal M. Vadile, Jayshree P. Godhaniya, Chirag Jadav, Ajay I. Patel, Ashok B. Patel, Ashvin Dudhrejiya, Sunny Shah, Urvi J. Chotaliya, Devang Sheth · 发表于：Research Journal of Pharmacy and Technology · 年份：2024 · DOI：10.52711/0974-360x.2024.00668 · 被引用次数：1 · 研究领域：Analytical Methods in Pharmaceuticals、Analytical Chemistry and Chromatography、Drug Solubulity and Delivery Systems

This study outlines a method using RP-HPLC to analyze Rosuvastatin calcium and Teneligliptin hydrobromide hydrate. The separation was accomplished at λ(245nm) with a 1mL/min F.R with [KH2PO4 buffer (pH 3): Methanol: Acetonitrile (75:20:5 %v/v)] using a C18 (25 cm x 4.6 mm, 5µm). Both were eluted with retention times of 3.19 min and 2.08 min respectively. Linearity was observed across a defined concentration range for both drugs, with high correlation coefficients. Accuracy of the method was confirmed through analysis of known concentrations, indicating its suitability for routine analysis of these compounds in a synthetic mixture.