Interstitial Cells of Cajal Are Required for Different Intestinal Motility Responses Induced by Acupuncture

作者：Longhua Du, Qing-Guang Qin, Xun He, Xiaoxi Wang, Guang Sun, Bing Zhu, Kun Liu, Xinyan Gao · 发表于：Neurogastroenterology & Motility · 年份：2024 · DOI：10.1111/nmo.14973 · 被引用次数：8 · 研究领域：Gastrointestinal motility and disorders、Acupuncture Treatment Research Studies、Vagus Nerve Stimulation Research

ABSTRACT Background The movement of intestinal smooth muscle is regulated by the external autonomic nervous system (ANS) and its internal enteric nervous system (ENS). Previous studies have shown that acupuncture has a bidirectional regulating effect on intestinal motility through the sympathetic and vagal ANSs. ENS can independently regulate the sensory, secretory, and motor functions of the intestine. The interstitial cells of Cajal (ICC), the pacemaker cells in ENS, play a key role in maintaining gastrointestinal motility. However, studies on the role and mechanism of ICC in the regulation of intestinal function by acupuncture are still unclear. Methods To investigate the effect of ICC on the regulation of intestinal motility by manual acupuncture (MA), we recorded the pressure of warm water‐filled manometric balloons in duodenum, jejunum, and distal colon in ICC deficiency WsWs −/− rats and wild‐type littermates WsRC +/+ rats, and performed MA at ST25 (Tianshu), ST37 (Shangjuxu), LI11 (Quchi), and BL25 (Danchangshu) acupoints. Furthermore, the excretion of phenol red in feces before and after MA at ST37 or ST25 was assessed. Key Result In WsRC +/+ rats, MA at ST37, LI11, and BL25 promoted duodenal, jejunal, and distal colon motility, whereas MA at ST25 significantly inhibited duodenal and jejunal motility and promoted distal colon motility. ICC deficiency in WsWs −/− rats led to a reduction in the promoting effect of LI11 on duodenal motility, a decrease in the promoting ...