18F-5-fluoro-aminosuberic acid PET/CT imaging of oxidative-stress features during the formation of DEN-induced rat hepatocellular carcinoma

作者：Feng Xiong, Yilin Yang, Zheyi Han, Buchuan Zhang, Kijung Kwak, Pei Wang, Qiaorong Chen, Ziqiang Wang, Jingfei Yang, Xiaoyun Deng, Sijuan Zou, Zhuoli Zhang, Pengtao You, Bo Yu, Xiaohua Zhu · 发表于：Theranostics · 年份：2024 · DOI：10.7150/thno.100467 · 被引用次数：4 · 研究领域：Medical Imaging Techniques and Applications、Radiopharmaceutical Chemistry and Applications

Rationale:The role of oxidative stress metabolism during hepatocellular carcinoma (HCC) formation potentially allows for positron emission tomography (PET) imaging of oxidative stress activity for early and precise HCC detection.However, there is currently limited data available on oxidative-stress-related PET imaging for longitudinal monitoring of the pathophysiological changes during HCC formation.This work aimed to explore PET-based longitudinal monitoring of oxidative stress metabolism and determine the sensitivity of [18F]-5-fluoroaminosuberic acid ([18F]FASu) for assessing pathophysiological processes in diethylnitrosamine (DEN) induced rat HCC.Methods: Genomic and clinical data were obtained from the HCC dataset (n = 383) in The Cancer Genome Atlas (TCGA-LIHC) and Gene Expression Omnibus (GEO) datasets.Wistar rats were administered DEN weekly, either by gavage (i.g.) at doses of 10 mg/kg or 80 mg/kg or by intraperitoneal injection (i.p.) at 80 mg/kg, with continuous modeling over a 12-week period followed by 24 weeks of consecutive feeding.PET/CT imaging was conducted at weeks 8, 15, and 21 by tail vein injections of [18F]FASu and [18F]FDG (~3.7 MBq).Finally, contrast-enhanced CT imaging of the nodules was performed at the designed time point.The rats in each group were sacrificed at multiple time points to perform a correlation analysis between PET imaging findings and histological examinations.Results: Bioinformatics analysis revealed that upregulation expression of ...