Probing Deep Hydrogen Polysulfides Fluctuations In Vivo by Engineering Activatable Fluorescence Reporters with Second Near-Infrared Window Emission

作者：Lixin Sun, Zhecha Zheng, Ziwen Zhang, Kehuan Wu, Hongyu Wu, Huili Liu, Mo Wang, Chunchang Zhao, Xianfeng Gu · 发表于：Analytical Chemistry · 年份：2024 · DOI：10.1021/acs.analchem.4c04848 · 被引用次数：16 · 研究领域：Sulfur Compounds in Biology、Industrial Gas Emission Control、Atmospheric chemistry and aerosols

Deep and comprehensive understanding of the intricate biochemical processes mediated by H 2 S n ( n ≥ 1), but not H 2 S, is of paramount importance. A few fluorescent probes have been developed with the intention of addressing this issue. However, there is currently no evidence of any activatable NIR-II fluorescent probes for H 2 S n -specific imaging over H 2 S. In this study, we developed the inaugural H 2 S n -activated NIR-II probe for specific and deep imaging of H 2 S n through a series of molecular engineering strategies. Strong electron-absorbing moieties enabled the redshift of the emission of the designed reporter, while leaving groups with different dissociation capacities were responsible for modulating the selective and sensitive responsiveness to H 2 S n over H 2 S. By using this activatable specific probe for deep tissue mapping of endogenous H 2 S n fluctuations, accurate identification of acute inflammation in animal models was achieved. It is anticipated that the advancement of this activatable NIR-II probe will facilitate a more profound comprehension of the physiological implications of H 2 S n .