Deciphering potential molecular mechanisms in clear cell renal cell carcinoma based on the ubiquitin‐conjugating enzyme E2 related genes: Identifying UBE2C correlates to infiltration of regulatory T cells

作者：Xiaoqiang Feng, Zhenwei Wang, Meini Cen, Zongtai Zheng, Bangqi Wang, Zongxiang Zhao, Zhihui Zhong, Yesong Zou, Qian Lv, Shiyu Li, Li‐Min Huang, Hai Huang, Xiaofu Qiu · 发表于：BioFactors · 年份：2024 · DOI：10.1002/biof.2143 · 被引用次数：7 · 研究领域：Renal cell carcinoma treatment、Ferroptosis and cancer prognosis、Renal and related cancers

Renal clear cell carcinoma (ccRCC) is a highly aggressive and common form of kidney cancer, with limited treatment options for advanced stages. Recent studies have highlighted the importance of the ubiquitin-proteasome system in tumor progression, particularly the role of ubiquitin-conjugating enzyme E2 (UBE2) family members. However, the prognostic significance of UBE2-related genes (UBE2RGs) in ccRCC remains unclear. In this study, bulk RNA-sequencing and single-cell RNA-sequencing data from ccRCC patients were retrieved from the Cancer Genome Atlas and Gene Expression Omnibus databases. Differential expression analysis was performed to identify UBE2RGs associated with ccRCC. A combination of 10 machine learning methods was applied to develop an optimal prognostic model, and its predictive performance was evaluated using area under the curve (AUC) values for 1-, 3-, and 5-year overall survival (OS) in both training and validation cohorts. Functional enrichment analyses of gene ontology and Kyoto Encyclopedia of Genes and Genomes were conducted to explore the biological pathways involved. Correlation analysis was conducted to investigate the association between the risk score and tumor mutational burden (TMB) and immune cell infiltration. Immunotherapy and chemotherapy sensitivity were assessed by immunophenoscore and tumor immune, dysfunction, and exclusion scores to identify potential predictive significance. In vitro, knockdown of the key gene UBE2C in 786-O cells by spec...