Nasal, dermal, oral and indoor dust microbe and their interrelationship in children with allergic rhinitis

作者：Hao Tang, Shuang Du, Zhiping Niu, Dongjun Zhang, Zhiwei Tang, Han Chen, Zhuoru Chen, Mei Zhang, Yanyi Xu, Yu Sun, Xi Fu, Dan Norbäck, Jie Shao, Zhuohui Zhao · 发表于：BMC Microbiology · 年份：2024 · DOI：10.1186/s12866-024-03668-9 · 被引用次数：8 · 研究领域：Allergic Rhinitis and Sensitization、Pediatric health and respiratory diseases、Indoor Air Quality and Microbial Exposure

BACKGROUND: Allergic rhinitis (AR) subjects might have their microenvironment changed due to pathogenesis and living environment. Whether the nasal microbe in AR children differs from healthy subjects and how it interplays with dermal, oral and indoor dust microbe needs to be elucidated. METHODS: In this case-control study, we analyzed and compared the bacterial characterization and associations in nasal, dermal, oral swab samples and dust samples in 62 children with physician-diagnosed AR(cases) and 51 age- and gender-matched healthy ones with no history of allergic diseases(controls). Full-length 16S rRNA sequencing(swabs) and shotgun metagenomics(dust) were applied. Bacterial diversity, composition, abundance difference characteristics and fast expectation-maximization for microbial source tracking(FEAST) analysis were performed and compared between cases and controls. RESULTS: The α-diversity of dust microorganisms in AR was lower than that in control group (P = 0.034), and the β-diversity indices of microorganisms in nasal cavity (P = 0.020), skin (P = 0.001) and dust (P = 0.004) were significantly different from those in control group. At species levels, a total of 10, 15, 12, and 15 bacterial species were differentially enriched in either cases or controls in nasal, dermal, oral, and dust samples, respectively(Linear Discriminant Analysis(LDA) score > 2, P < 0.05). Staphylococcus epidermidis was the single species simultaneously more abundant in nasal, dermal and dust ...