Ferroptosis is involved in the damage of ocular lens under long-term PM2.5 exposure in rat models and humans

作者：Feiyin Sheng, Yuzhou Gu, Shengjie Hao, Ye Liu, Shuying Chen, Bing Lu, Lü Chen, Wei Zhao, Di Wu, Yili Xu, Rongrong Chen, Yu Han, Xiaoming Lou, Xiaofeng Wang, Zhijian Chen, Ke Yao, Qiuli Fu · 发表于：Ecotoxicology and Environmental Safety · 年份：2024 · DOI：10.1016/j.ecoenv.2024.117397 · 被引用次数：11 · 研究领域：Air Quality and Health Impacts、Heme Oxygenase-1 and Carbon Monoxide、Climate Change and Health Impacts

Epidemiological studies show a positive association between air pollution and age-related cataracts, but the pathogenic mechanism remains unclear. This study first demonstrates that fine particulate matter (PM 2.5 ) induces ferroptosis in the lens, leading to morphological and functional disorders, through human, animal, and cellular samples. In 3-week PM 2.5 -exposed rat models (10 µl 1 mg/ml PM 2.5 suspension per eye, 4 times a day), we find that many vacuoles form in the lens equatorial region by analysis of haematoxylin and eosin staining after PM 2.5 exposure. Using iron and glutathione (GSH) assay kits, we found increased Fe 2+ contents and decreased GSH levels in PM 2.5 -exposed rats’ lenses. Additionally, the lipid peroxide 4-hydroxynonenal (4-HNE) was also found to be elevated with immunoblot, suggesting ferroptosis is involved. Ferroptosis was also observed in human lens epithelial cells treated with 25, 50, and 100 µg/ml PM 2.5 suspension for 24 h, accompanied by decreased cell viability and migration. Furthermore, we collect about 60 human lens anterior capsule (HLAC) samples for RNA-seq. The results show that compared to HLACs from areas with PM 2.5 concentration ≤30 μg/m³, ferroptosis-related genes expression of those from areas with PM 2.5 concentration ≥35 μg/m³ are significantly altered, such as glutathione peroxidase 4 and STEAP family member 3. Also, human lens in areas with high PM 2.5 concentrations showed elevated levels of transferrin receptor and 4-HNE...