A study on the function of methionine in growth, immunity, antioxidant, endoplasmic reticulum stress and apoptosis of largemouth bass (Micropterus salmoides)

作者：Hualiang Liang, Dongyu Huang, Mingchun Ren, Lu Zhang, Haifeng Mi, Ahmed M. Aboseif, Chunyu Xue, Jiaze Gu · 发表于：Aquaculture Reports · 年份：2024 · DOI：10.1016/j.aqrep.2024.102531 · 被引用次数：7 · 研究领域：Aquaculture Nutrition and Growth、Aquaculture disease management and microbiota、Protein Hydrolysis and Bioactive Peptides

An 8-week culture experiment was conducted to evaluate the effects of methionine (Met) on growth, immunity and antioxidant capacity in largemouth bass ( Micropterus salmoides ). Six dietary Met levels were tested and formulated based on isonitrogenous and isoenergetic principles: 0.74 %, 0.91 %, 1.13 %, 1.37 %, 1.59 % and 1.75 %. Compared to the control group (0 % Met supplementation, diet level of 0.74 %), dietary Met supplementation significantly improved growth, as indicated by higher FW, WG, SGR and lower FCR ( p < 0.05). Furthermore, liver Oil Red O staining revealed a significantly lower positive area for groups receiving 1.13–1.75 % Met ( p < 0.05). Plasma TC and TG were also significantly lower in the 0.91 %-1.75 % dietary Met groups ( p < 0.05). Met supplementation enhanced antioxidant capacity by increasing plasma levels of antioxidant parameters, including CAT, T-SOD and GSH, and by modulating antioxidant gene expression within the Nrf2 signalling pathway in the liver ( nrf2 , keap1 , cat and sod ) ( p < 0.05), which was supported by significantly lower MDA level in plasma ( p < 0.05). Met supplementation also alleviated apoptosis and endoplasmic reticulum stress (ERS) by upregulating anti-apoptotic genes ( bcl-2 , bcl-xl ) and downregulating pro-apoptotic and ERS marker genes ( caspase 3 , bax , caspase 8 , atf4 , caspase 9 , ask1 , ire1 , chopα , eif2α , perk ) ( p < 0.05). Met supplementation also reduced inflammatory responses by modulating NF-κB signalling pat...