Multi-omics analysis reveals indicator features of microbe-host interactions during Candida albicans colonization and subsequent infection

作者：Huan Zhang, Daoyuan Song, Qiulin Luo, Jiangkun Yu, Yingpu Wei, Di Chen, Guangjuan Wu, Zhi Zhang, Li Zhao, Hongchao Jiang, Jingquan Gan, De‐Yao Deng, Hui Li, Wenli Yuan · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1476429 · 被引用次数：6 · 研究领域：Antifungal resistance and susceptibility、Gut microbiota and health、Probiotics and Fermented Foods

Introduction Candida albicans gastrointestinal (GI) colonization is crucial for the onset of invasive disease. This research encompassed 31 patients diagnosed with Candida spp. bloodstream infections during their admission to a university hospital in China. Methods We explored risk factors associated with C. albicans GI colonization and ensuing translocated infection. Animal models were established via gavage with clinical isolates of C. albicans to induce GI tract colonization and subsequent kidney translocation infection. Our analysis is focused on 16S rRNA gene sequencing, metabolomics of colon contents, and transcriptomics of colon tissues, examining the intestinal barrier, inflammatory responses, and immune cell infiltration. Results This study observed that down-regulation of programmed cell death 1 (PD-1) in colon tissues is likely linked to the progression from C. albicans colonization to translocated infection. Notably, reductions in Dubosiella abundance and Short-chain fatty acids (SCFA) levels, coupled with increases in Mucispirillum and D-erythro-imidazolylglycerol phosphate, were indicator features during the advancement to translocated invasive infection in hosts with rectal colonization by C. albicans and lower serum protein levels. Conclusion Given the similarity in intestinal bacterial communities and metabolome profiles, antifungal treatment may not be necessary for patients with nonpathogenic C. albicans colonization. The reduced expression of PD-1 in colon...