Patient-derived tumor organoid and fibroblast assembloid models for interrogation of the tumor microenvironment in esophageal adenocarcinoma

作者：Benjamin P. Sharpe, Liliya Nazlamova, Carmen Ka Man Tse, David Annandale Johnston, Jaya Thomas, Rhianna R R Blyth, Oliver J. Pickering, Ben L. Grace, Jack Harrington, Rushda Rajak, Matthew J.J. Rose-Zerilli, Zoë S. Walters, Tim Underwood · 发表于：Cell Reports Methods · 年份：2024 · DOI：10.1016/j.crmeth.2024.100909 · 被引用次数：21 · 研究领域：Cancer Cells and Metastasis、Mathematical Biology Tumor Growth、Cancer Genomics and Diagnostics

The tumor microenvironment (TME) comprises all non-tumor elements of cancer and strongly influences disease progression and phenotype. To understand tumor biology and accurately test new therapeutic strategies, representative models should contain both tumor cells and normal cells of the TME. Here, we describe and characterize co-culture tumor-derived organoids and cancer-associated fibroblasts (CAFs), a major component of the TME, in matrix-embedded assembloid models of esophageal adenocarcinoma (EAC). We demonstrate that the assembloid models faithfully recapitulate the differentiation status of EAC and different CAF phenotypes found in the EAC patient TME. We evaluate cell phenotypes by combining tissue-clearing techniques with whole-mount immunofluorescence and histology, providing a practical framework for the characterization of cancer assembloids.