Sodium Iodate: Rapid and Clinically Relevant Model of AMD

作者：Jasmine Geathers, Stephanie L. Grillo, Ema Karakoleva, Gregory P. Campbell, Yong Du, Han Chen, Alistair J. Barber, Yuanjun Zhao, Jeffrey M. Sundstrom · 发表于：Frontiers in Bioscience-Landmark · 年份：2024 · DOI：10.31083/j.fbl2911380 · 被引用次数：7 · 研究领域：Retinal Diseases and Treatments、Retinal Development and Disorders、Ophthalmology and Visual Impairment Studies

Background: Age-related macular degeneration (AMD) is the most common cause of vision loss in people above the age of 50, affecting approximately 10% of the population worldwide and the incidence is rising. Hyperreflective foci (HRF) are a major predictor of AMD progression. The purpose of this study was to use the sodium iodate mouse model to study HRF formation in retinal degeneration. Methods: Sodium iodate (NaIO3) treated rodents were studied to characterize HRF. 3-month-old male wild-type (WT) C57Bl/6J mice were injected with phosphate-buffered saline (PBS) or varying doses of NaIO3 (15–60 mg/kg). Optical Coherence Tomography (OCT) images were collected at baseline and several days post-NaIO3 injection. Retinal thicknesses were measured using Bioptigen software. Seven days post-injection, eyes were prepared for either transmission electron microscopy (TEM), Hematoxylin & Eosin (H&E), or immunofluorescence. Results: OCT imaging of the mice given higher doses of NaIO3 revealed HRF formation in the neural retina (n = 4). The amount of HRF correlated with the degree of retinal tissue loss. H&E and TEM imaging of the retinas seven days post-NaIO3 injection revealed several pigmented bodies in multiple layers of the retina (n = 3–5). Immunofluorescence revealed that some pigmented bodies were positive for macrophage markers and an epithelial-to-mesenchymal transition marker, while all were retinal pigment epithelium (RPE) 65-negative (n = 4). Conclusions: The data ...