Amino acid metabolic shifts in rice: responses to elevated CO2, cyanide, and nitrogen sources

作者：Chengzhi Li, Abid Ullah, Yi Kang, Xiao-Zhang Yu · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2024 · DOI：10.1186/s40538-024-00701-x · 被引用次数：5 · 研究领域：Cassava research and cyanide、GABA and Rice Research、Plant Micronutrient Interactions and Effects

Amino acids (AAs) play multiple roles in plant development, and their reorientation is crucial strategy for plants in metabolic adaptation to various abiotic stresses. The incorporation of exogenous CN− into the N fertilization in plants is evident, wherein elevated CO2 increases utilization and assimilation of biodegradable N-containing pollutants, consequently reduce phytotoxicity. In this study, a hydroponic system was employed to investigate the effects of different nitrogen (N) forms (nitrate: NO3− and ammonium: NH4+), CO2 concentrations (ambient at 350 ppm and elevated at 700 ppm), and exogenous cyanide (KCN at 3.0 mg CN/L) on rice plants using metabonomics analysis. Elevated [CO2] (700 ppm) significantly enhanced the growth rate of rice seedlings, particularly under NH4+ nutrition combined with CN− treatment, compared to ambient [CO2] (350 ppm). Under elevated [CO2] both NO3− and NH4+-fed plants exhibited significantly higher CN− uptake and assimilation rates, with NH4+-fed plants showing a greater response. Metabolomic analysis revealed distinct alteration in AA profiles, where elevated [CO2] and exogenous CN− significantly influenced the proportions of the glutamate (Glu) pathway and aspartate (Asp) pathway under both N treatments. Notably, NH4+-fed plants under CN− stress demonstrated a 5.75-fold increase in total AA content in shoots under elevated [CO2], while NO3−-fed plants CN− stress showed a smaller increase of 1.81-fold. These results suggest that elevated [C...