Diversity and transmissibility of RNA viruses in the small brown planthopper, Laodelphax striatellus

作者：Qianzhuo Mao, Zhuang-Xin Ye, Jing-Na Yuan, Chao Ning, Meng-Nan Chen, Zhongtian Xu, Yu-Hua Qi, Yan Zhang, Ting Li, Yujuan He, Gang Lü, Hai‐Jian Huang, Jia‐Bao Lu, Ji‐Chong Zhuo, Qing‐Ling Hu, Chuan‐Xi Zhang, Jianping Chen, Jun‐Min Li · 发表于：Journal of Virology · 年份：2024 · DOI：10.1128/jvi.00191-24 · 被引用次数：8 · 研究领域：Plant Virus Research Studies、Insect-Plant Interactions and Control、Phytoplasmas and Hemiptera pathogens

ABSTRACT While a considerable number of viruses have been recently discovered in hematophagous insects, there remains insufficient research on virus diversity and their association with phytophagous insect hosts. In this study, we conducted a systematic investigation of the RNA virome in the small brown planthopper (SBPH), Laodelphax striatellus , an important vector of plant viruses. We successfully identified a total of 22 RNA viruses, including 17 novel viruses, from various families. These viruses were prevalent and abundant in SBPH populations, originating from the lab or field, with +ssRNA viruses composing the core SBPH viruses. Subsequent analysis revealed that the overall abundance of RNA viruses in SBPH remained relatively consistent across different developmental stages of the insects, although the titers of individual viruses varied among different insect tissues. This indicates a delicate balance between the viruses and their insect hosts. Interestingly, cross-species experiments confidently indicated that certain SBPH viruses could successfully infect and replicate in two other rice planthopper species (the brown planthopper and the white-backed planthopper) through microinjection. In conclusion, this study provides valuable insights into the RNA virome and its adaptability in a phytophagous insect, contributing to a better understanding of the intimate relationship between viruses and host insects. IMPORTANCE In the last decade, advances in the next-generation ...