Overexpression of StCDPK13 in Potato Enhances Tolerance to Drought Stress

作者：Zhenzhen Bi, Simon Dontoro Dekomah, Yihao Wang, Zhuanfang Pu, Xiangdong Wang, Richard Dormatey, Chao Sun, Yuhui Liu, Zhen Liu, Jiangping Bai, Panfeng Yao · 发表于：International Journal of Molecular Sciences · 年份：2024 · DOI：10.3390/ijms252312620 · 被引用次数：9 · 研究领域：Potato Plant Research、Plant nutrient uptake and metabolism、Plant Pathogens and Resistance

Calcium-dependent protein kinases (CDPKs), which are activated by transient changes in the Ca2+ concentration in plants, are important for various biological processes, such as growth, development, defense against biotic and abiotic stresses, and others. Mannitol is commonly used as an osmotic regulatory substance in culture medium or nutrient solutions to create water-deficit conditions. Here, we cloned the potato (Solanum tuberosum L.) StCDPK13 gene and generated stable transgenic StCDPK13-overexpression potato plants. To investigate the potential functions of StCDPK13 in response to drought stress, overexpression-transgenic (OE1, OE2, and OE7) and wild-type (WT) potato seedlings were cultured on MS solid media without or with mannitol, representing the control or drought stress, for 20 days; the elevated mannitol concentrations (150 and 200 mM) were the drought stress conditions. The StCDPK13 gene was consistently expressed in different tissues and was induced by drought stress in both OE and WT plants. The phenotypic traits and an analysis of physiological indicators revealed that the transgenic plants exhibited more tolerance to drought stress than the WT plants. The overexpression lines showed an increased plant height, number of leaves, dry shoot weight, root length, root number, root volume, number of root tips, fresh root weight, and dry root weight under drought stress. In addition, the activities of antioxidant enzymes (CAT, SOD, and POD) and the accumulation of pr...