Exploring the role of miR-125b-5p as a pro-inflammatory factor in Alzheimer's disease pathology

作者：Wenjia Liu, Sophia Chen, Xin Rao, Xiaodong Chen, Liyang Yu, Jiangtao Zhang, Jiong Chen, Bohan Cui · 发表于：Journal of Alzheimer s Disease · 年份：2024 · DOI：10.1177/13872877241297178 · 被引用次数：5 · 研究领域：MicroRNA in disease regulation、Alzheimer's disease research and treatments、Neuroinflammation and Neurodegeneration Mechanisms

Background Alzheimer's disease (AD) is a common neurodegenerative disease, where neuroinflammation significantly influences its pathophysiology by driving the disease's pathological cascade. As a pro-inflammatory regulator, miR-125b-5p contributes to AD progression, though its precise role and mechanisms remain unclear. Objective We aims to identify mRNAs significantly regulated by pro-inflammatory miR-125b-5p in AD and uncover key neuroinflammatory pathways. Methods Target mRNAs regulated by miR-125b-5p were predicted using online databases and analyzed with two mRNA datasets to identify differentially expressed mRNAs (DEmRNAs). Enrichment analysis was conducted to explore their biological functions and pathways. The significance of DEmRNAs expression in AD-related inflammatory pathways was verified by the Wilcoxon test, predictive accuracy was assessed via area under the curves (AUCs), and novel mRNAs were identified through positive control analysis. Results A total of 613 miR-125b-5p target mRNAs were identified, and 44 DEmRNAs were detected to be regulated by miR-125b-5p in two datasets. The 44 target DEmRNAs associated with AD include three key pathways: insulin signaling ( EXOC7, FLOT2, MKNK2), phosphatidylinositol signaling ( IP6K1, MTMR3), and phospholipase D signaling ( CYTH1, GAB2). Correlation analysis indicated strong correlations among 7 mRNAs, all showing significant differential expression, with AUCs above 0.5, confirming their predictive value. Three mRNAs ( ...