Engineering Artificial Three-Species Microbial Consortium to Produce High-Power Bioelectricity from Discarded Cellulosic Biomass of Liquor Industry

作者：Rui Tang, Baocai Zhang, Longhai Dai, Yiyun Wang, Guosheng Xin, Zhanying Liu, Feng Li, Hao Song · 发表于：ACS Sustainable Chemistry & Engineering · 年份：2024 · DOI：10.1021/acssuschemeng.4c04119 · 被引用次数：11 · 研究领域：Microbial Fuel Cells and Bioremediation、Microbial Community Ecology and Physiology、Modular Robots and Swarm Intelligence

Converting cellulose and other widely distributed biomass resources into bioelectricity by exoelectrogen-based bioelectrochemical systems has been developed as a potential solution to the simultaneous environmental pollution treatment and power harvest. However, exoelectrogens are generally unable to utilize cellulose for cell growth and power generation due to a lack of relevant metabolic pathways and enzymes, which considerably limited practical applications. In this study, to use discarded cellulosic biomass from liquor industry as carbon source for power generation, we constructed an artificial three-species microbial consortium that included Trichoderma reesei, Lactobacillus pentosus, and Shewanella oneidensis . In this consortium, T. reesei digested cellulose into soluble sugars, which were then converted to lactate by L. pentosus, and S. oneidensis subsequently utilized lactate as the carbon source and electron donor to produce electricity. To enhance the energy conversion efficiency of this consortium, we first optimized the T. reesei – L. pentosus subconsortium, including the inoculum size and reaction temperature and pH, to increase degradation of cellulose and production of lactate, which led to an output power density of 40.39 mW/m 2 by the artificial three-species microbial consortium. Second, to improve the extracellular electron transfer rate and biofilm formation of S. oneidensis, the flavin synthesis and extracellular polysaccharide accumulation were strength...