Down-regulation of TAGLN2 associated with the development of preeclampsia by effecting the Rap1 signaling pathway

作者：Ping Yang, Xinyang Liu, Jinli Lyu, Qiaoli Feng, Yuzhen Ding, Shilin Zhong, Ping Liu, Yiheng Liang, Chun‐Feng Liu, Liting Huang, Pingyue Zhao, Xiu‐Qing Li, Kaidong Ma, Shangrong Fan, Xiaowei Zhang · 发表于：Placenta · 年份：2024 · DOI：10.1016/j.placenta.2024.11.009 · 被引用次数：4 · 研究领域：Pregnancy and preeclampsia studies、Reproductive System and Pregnancy、Preterm Birth and Chorioamnionitis

INTRODUCTION: Preeclampsia (PE) poses significant global challenges to pregnancy health, being a leading cause of maternal and perinatal morbidity and mortality. Unfortunately, effective treatment options remain limited, necessitating the urgent development of novel therapeutic strategies. This study is to investigate down-regulation of Transgelin-2 (TAGLN2) contributes to the development of PE through suppression of the Rap1 signaling pathway. METHODS: Placentas from PE patients were collected for a transcriptome analysis. Down-regulation experiments of TAGLN2 were performed in mouse and HTR-8/SVneo cells to generate PE models. The mechanism by which down-regulation of TAGLN2 induces PE was explored based on these PE model through transcriptome and proteome analysis and molecular tests. RESULTS: Our findings revealed that the expression levels of Rap1A was significantly reduced in the placenta of PE patients. The expression level of Rap1A in the placental tissue of sh_Tagln2 PE model mice is down-regulated. In addition, TAGLN2 down-regulation impede the proliferation and migration of HTR8/SVneo cells and lead to the decreased expression of Rap1A. Meanwhile, Rap1A down-regulation impede both the proliferation and migration of HTR8/SVneo cells. Both transcriptomic and proteomic levels of sh-TG2 HTR8/SVneo cells demonstrated Rap1 signaling pathway and related key genes was inhibited after TAGLN2 down-regulation. CONCLUSION: Our results confirm that down-regulation of TAGLN2 in ...