Yeast cell wall extract as a strategy to mitigate the effects of aflatoxin B1 and deoxynivalenol on liver and intestinal health, and gut microbiota of largemouth bass (Micropterus salmoides)

作者：Zhe Yu, Zhen Zhang, Tsegay Teame, Leluo Guan, Ruiguo Wang, Ronghua Zhu, Qingshuang Zhang, Hongwei Yang, Na Cui, Yuan Huang, Chao Ran, Yalin Yang, Xiaoou Su, Zhigang Zhou · 发表于：Aquaculture · 年份：2024 · DOI：10.1016/j.aquaculture.2024.741917 · 被引用次数：21 · 研究领域：Meat and Animal Product Quality、Aquaculture Nutrition and Growth、Animal Nutrition and Physiology

The increasing use of plant-based raw materials in aquatic feed has led to a growing concern about the harmful effects of mycotoxins on aquatic animals. This study aimed to evaluate the supplementation of yeast cell wall extract (YCWE) on the growth performance, liver and intestine health, and gut microbiota of largemouth bass ( Micropterus salmoides ) fed with aflatoxin B1 (AFB1), and deoxynivalenol (DON) contaminated feeds. AFB1, DON and YCWE were supplemented to the basic formula feed at different concentrations to develop eight dietary groups and M. salmoides were fed for 8 weeks. Results showed that the inclusion of YCWE in mycotoxins-contaminated diets had no effect on the growth performance but significantly affected the immunity and antioxidant capacity of M. salmoides ( P < 0.05). The overall changes in serum biochemical indicators were not significant, except that the ALT/AST activities were significantly higher ( P < 0.05) under the mycotoxin challenge, and significantly lower in 0.2 % YCWE treatment group. Furthermore, the expression of genes related to liver and intestine inflammation (pro-inflammatory factors nf-kb, tnf-a, il-1β, il-6, il-8, ifn-γ and anti-inflammatory factors il-10, tgf-β ), and antioxidant regulatory factors nrf2/keap1 showed an up-regulation trend, and the content of the lipid metabolite MDA was notably higher under mycotoxin challenge and YCWE treatments ( P < 0.05). Moreover, the supplementation of YCWE significantly alleviated the inflamma...