Eugenol targets laccase Cglac4 to subvert the pathogenicity of Colletotrichum gloeosporioides and control the postarvest fruit anthracnose

作者：Qing Tan, Jinji Pu, Mengting Zhang, Zhuoli Chen, Xi Li, Zhengjie Zhu, He Zhang · 发表于：Postharvest Biology and Technology · 年份：2024 · DOI：10.1016/j.postharvbio.2024.113319 · 被引用次数：8 · 研究领域：Plant Pathogens and Fungal Diseases、Mycorrhizal Fungi and Plant Interactions、Enzyme-mediated dye degradation

Eugenol has a high antifungal activity against filamentous fungi , so it is essential to elucidate the underlying mechanisms of eugenol controling disease. However, the mechanism underlying the target protein of eugenol is largely unclear. Here, we revealed that eugenol efficiently suppressed the growth and development of Colletotrichum gloeosporioides , the pathogen of postharvest fruit anthracnose , which caused significant economic losses. Moreover, we identified the target protein of eugenol in C. gloeosporioides-Cglac4 , which positively regulated pathogenicity via inhibited the conidial germination and appressorium formation. Taken together, the identification of the eugenol-Cglac4 pair module not only illustrates a novel mechanism by which eugenol subvert the laccase activity of C. gloeosporioides to orchestrate the pathogenicity but also provides a potential application in the green management of postharvest anthracnose.