Whole genome sequencing revealed high proportions of ST152 MRSA among clinical Staphylococcus aureus isolates from ten hospitals in Ghana

作者：Beverly Egyir, Christian Owusu-Nyantakyi, Alfred Bortey, Grebstad Rabbi Amuasi, Felicia Owusu, William Boateng, Hawawu Ahmed, Justice Kwesi Danso, Agnes Oclu, Quaneeta Mohktar, Georgina Tetteh-Ocloo, Harold K. Amegbletor, Kwabena Fosu, Francis Tetteh, Solomon Asante-Sefa, Oliver Nangkuu Deberu, Kennedy Mensah Osei, Joana Twasam, Sarkodie Kodom, Esther Gyinae, James Sampah, Nicholas Dzifa Dayie, Noah Obeng‐Nkrumah, William Addo Mills-Pappoe, Gifty Boateng, Pernille Nilsson, Harriet Affran Bonful, Bright Adu, René S. Hendriksen · 发表于：mSphere · 年份：2024 · DOI：10.1128/msphere.00446-24 · 被引用次数：11 · 研究领域：Antimicrobial Resistance in Staphylococcus、Bacterial Identification and Susceptibility Testing、Bacterial biofilms and quorum sensing

ABSTRACT Previous studies in Ghana indicated low prevalence of methicillin-resistant Staphylococcus aureus (MRSA) and predominance of ST152 methicillin-susceptible S. aureus (MSSA) among clinical isolates. ST152 MRSA clones are associated with severe infections and epidemics. Using whole genome sequencing (WGS), 159 S . aureus isolated from clinical sources (wound, blood, urine, ear, abscess, umbilical cord, eye, vaginal samples, and others) from 10 hospitals across Ghana were investigated. mecA (gene for methicillin resistance) was detected in 38% of the isolates. Panton–Valentine leucocidin toxin (PVL) gene occurred in 65% isolates, with 84% of the MRSA’s harboring the PVL gene. ST152 was the major clone, with 74% harboring the mecA gene. Other MRSA clones detected were ST5, ST5204, ST852, and ST1. MSSA clones included ST3249, ST152, ST5, ST1, and ST8. Twenty-three genes encoding resistance to 12 antimicrobial classes were observed with blaZ (97%) being the most prevalent. Other predominant resistance genes included tetK (46%), cat (42%), and dfrG (36%) encoding resistance for tetracyclines, phenicols, and diaminopyrimidine, respectively. Virulence genes for enterotoxins, biofilms, toxic-shock-syndrome toxins, hemolysins , and leukotoxins were also detected. Phylogenetic analysis revealed a shift in the dominant clone from MSSA ST152 to MRSA ST152 over the past decade. The study provides valuable insights into the genomic content of S. aureus from clinical sources in Ghana....