IL-10 mediates pleural remodeling in systemic lupus erythematosus

作者：Qian Niu, Limei Liang, Shu-Yi Ye, Chen-Yue Lian, Qian Li, Xiao Feng, Shuai-Jun Chen, Meng Wang, Yuan-Yi Zheng, Xiao-Lin Cui, Liqin Zhao, Zi-Heng Jia, Shihe Hu, Peipei Cheng, Pengcheng Cai, Hong Ye, Wanli Ma · 发表于：Cell Communication and Signaling · 年份：2024 · DOI：10.1186/s12964-024-01911-4 · 被引用次数：4 · 研究领域：Pleural and Pulmonary Diseases、T-cell and B-cell Immunology、Spondyloarthritis Studies and Treatments

BACKGROUND: Interleukin-10 (IL-10), a pivotal anti-inflammatory cytokine, has gotten attention for its involvement in tissue remodeling and organ fibrosis. Pleurisy and subsequent pleural remodeling are recognized as quantifiable indicators of systemic lupus erythematosus (SLE) activity. However, the role of IL-10 in SLE-associated pleural remodeling remains unknown. In this study, we investigated role of IL-10 in SLE-associated pleural remodeling and the underlying mechanism. METHODS: Clinical data and serum specimens were obtained from SLE patients, while pleural mesothelial cells and mouse models served as primary experimental subjects. The protein expression-related technologies, histopathological staining, and other experimental methods were used in the study. RESULTS: Our investigation got several key findings. Firstly, serum obtained from SLE patients with pleural thickening was found to induce pleural mesothelial cell remodeling. Subsequently, heightened levels of IL-10 were found in serum from SLE patients with pleural thickening compared to that of SLE patients without pleural thickening. Secondly, administration of recombinant IL-10 was confirmed its ability to induce pleural mesothelial cell remodeling, on the contrary, this remodeling was effectively mitigated by IL-10 inhibition. Notably, blockade of IL-10 significantly prevented collagen deposition and prevented thickening in pleura of SLE mouse models. Lastly, the IL-10/JAK2/STAT3/HIF1α/TMEM45A/P4HA1 signaling...