Detecting telomerase activity at the single-cell level using a CRISPR-Cas12a-based chip

作者：Yateng Jiang, Yanping Wang, Wen Luo, Xiaowei Luan, Zhibin Zhang, Yongchun Pan, Bangshun He, Yanfeng Gao, Yujun Song · 发表于：Lab on a Chip · 年份：2024 · DOI：10.1039/d4lc00619d · 被引用次数：14 · 研究领域：Telomeres, Telomerase, and Senescence、Advanced biosensing and bioanalysis techniques、CRISPR and Genetic Engineering

-cleavage activity of CRISPR-Cas12a. This amplification-based assay could be integrated with a microfluidic chip to enable highly sensitive detection of telomerase activity at the single-cell level, offering promising advancements in early cancer diagnosis.