Chromosome-level genome assembly and annotation of the tropical sea cucumber Stichopus monotuberculatus

作者：Ting Chen, Yun Yang, Xuan Wang, Qin Zhou, Zhenyu Xie, Dingding Fan, Chunhua Ren, Hongyan Sun, Peng Luo, Xiao Jiang, Long Hao, Chang Chen, Wenjie Pan, E Zixuan, Jiasheng Huang, Qianying Huang, Jianfeng Xu, Zepeng Zhang, Chuhang Cheng, Suzhong Yu, Yanhong Wang, Fajun Jiang, Aifen Yan, Chaoqun Hu · 发表于：Scientific Data · 年份：2024 · DOI：10.1038/s41597-024-03985-8 · 被引用次数：5 · 研究领域：Echinoderm biology and ecology、Aquatic life and conservation、Coral and Marine Ecosystems Studies

In this study, a chromosome-level genome of the tropical sea cucumber Stichopus monotuberculatus was generated by a combination of Nanopore long-read, Illumina short-read, and Hi-C sequencing technologies. The final assembly was 810.54 Mb in length, with contig N50 and scaffold N50 values of 10.15 Mb and 35.36 Mb, respectively. This assembly comprised 23 pseudo-chromosomes, covering 99.82% of the genome. Completeness analysis using BUSCO indicated that 97.8% of the metazoan conserved genes were presented in their entirety. A total of 29,596 protein-coding genes were predicted, with functional annotations available for 94.43% of these genes. The high-quality genome assembly produced in this study may provide an essential foundation for future researches on resource conservation and genetic breeding of S. monotuberculatus.