Assessing population genetic structure and diversity and their driving factors in Phoebe zhennan populations

作者：Jian Peng, Jiaxin Xie, Yunjie Gu, Hongying Guo, Shuaiying Zhang, Xin Huang, Xiandan Luo, Jianghong Qian, Minhao Liu, Xueqin Wan, Lianghua Chen, Xiong Huang, Fan Zhang, Fang He, Peng Zhu, Yu Zhong, Hanbo Yang · 发表于：BMC Plant Biology · 年份：2024 · DOI：10.1186/s12870-024-05810-1 · 被引用次数：12 · 研究领域：Genetic diversity and population structure、Plant Gene Expression Analysis、Genetic and Environmental Crop Studies

BACKGROUND: Phoebe zhennan, commonly known as "golden-thread nanmu," is one of the most valuable and protected tree species in China. An accurate understanding of the population genetic structure and its environmental factors is of significance for the protection and selection of new P. zhennan varieties. RESULTS: Sixteen nSSR and six cpSSR markers were used to determine the genetic diversity and population structure of P. zhennan and the effect of environmental factors on the genetic structure. The nSSR markers identified a total of 451 number of alleles (Na), while cpSSR markers detected 20 Na. A relative high level of genetic diversity was observed in the P. zhennan population evidenced by high Shannon's information index (I) of 0.671 and 2.294 based on cpSSR and nSSR datasets. The low value of fixation index (F) observed from the nSSR dataset indicated low breeding within the population. The genetic differentiation was mainly detected within populations (only 28% and 13% of the variance being between populations according to the nSSR and cpSSR datasets). Among them, the HNSZX (H = 0.469) and SCSZZ (I = 1.943) populations exhibited the highest level of genetic diversity, while the HNXXT (H = 0.041) and SCLJS (I = 0.943) populations exhibited the lowest level of genetic diversity. The average genetic differentiation coefficient (Fst) and gene flow (Nm) were 0.022-0.128 and 1.698-11.373, respectively, which indicated a moderate level of genetic differentiation and a high lev...