Effective removal of Pb from industrial wastewater: A new approach to remove Pb from wastewater based on engineered yeast

作者：Hao Luo, Zheng Su, Yang Liu, Dexian Yuan, Rui Wang, Yu-Hang Ning, D Zhang, Xianke Chen, Zhaobao Wang, Xue‐Yan Gao, Yue-Chao Zhang, Guang Cheng, Linxu Chen, Jianqun Lin · 发表于：Journal of Hazardous Materials · 年份：2024 · DOI：10.1016/j.jhazmat.2024.136516 · 被引用次数：15 · 研究领域：Adsorption and biosorption for pollutant removal、Heavy metals in environment、Chromium effects and bioremediation

The use of synthetic biology to construct engineered strains has provided new perspectives for addressing Pb contamination; however, the large-scale treatment of contaminants is still limited by high operating costs and technological constraints. This study introduces a novel technique for applying engineered yeast in the removal of heavy metals, offering a solution to the cost and process scale challenges associated with utilizing engineered yeast. Hydrogen sulfide-producing engineered yeast strains were constructed based on existing strategies by knocking out the gene encoding the O-acetyl-L-homoserine mercapturic enzyme, which plays a role in sulfate assimilation. To facilitate the transition of engineered yeast from laboratory settings to industrial applications while reducing operating costs and addressing process scale-up issues, we proposes a new operational technology for engineered yeast based on their mechanistic understanding and a response surface optimization approach. The development and application of low-cost engineered media provide important guidance for utilizing engineered yeast to tackle Pb-contaminated wastewater and for the production of PbS crystalline nanomaterials. The industrial culture system was designed using economical materials and, through the response surface methodology, achieved removal rates of 99.02 ± 0.06 % and 80.95 ± 9.68 % of Pb²⁺ from Pb acid electrolyte and industrial Pb wastewater, respectively. This study presents a new technologi...