Fast chlorophyll fluorescence rise kinetics as a high-throughput diagnostic tool for evaluating the capacity of 2-amino-3-methylhexanoic acid at inducing plant resistance against high temperature

作者：Jingjing Li, Haiou Liu, Yanjing Guo, Chang Yuan, Jing Zhang, He Wang, Qing Liu, Ji Yu, Zheng Zhang, Yujing Liu, Bernal E. Valverde, Shiguo Chen · 发表于：Environmental and Experimental Botany · 年份：2024 · DOI：10.1016/j.envexpbot.2024.106040 · 被引用次数：5 · 研究领域：Plant Growth Enhancement Techniques、Plant responses to elevated CO2、Plant Stress Responses and Tolerance

Plant resistant induction is considered as a promising strategy for protecting crops against extreme high temperature (HT). However, a high-throughput method to accurately estimate the capacity of plant resistance inducers (PRIs) for HT resistance has not been developed. Here, we present a simple approach using fast chlorophyll fluorescence kinetics in Arabidopsis leaf discs to assess PRI efficacy in inducing HT resistance. Both 2-amino-3-methylhexanoic acid (AMHA) and salicylic acid (SA) significantly alleviated the temperature-dependent increase in the K-peak of the OJIP curve and variations in amplitude of heat-responsive JIP-test parameters within the elevated-temperature range of 25–42℃. The PI ABS (performance index on absorption basis) and W K (relative variable fluorescence at the K-step to the amplitude F J - F O ) as two classical heat-responsive characteristic parameters were used to produce a novel hypersensitive parameter HT sensitivity indicator, PI ABS /W K (named H s ). Based on the correlation of log H s with elevated temperatures, a model for quantifying the capacity of HT-resistance induction (called C i ) by AMHA or SA was established. A three-grade classification according to the C i value was proposed as low (0< C i ≤ 1℃), moderate (1℃ < C i ≤ 2℃), and high res i stance ( C i > 2℃). AMHA at 1 µM and SA at 100 µM had C i values of 2.49℃ and 4.09℃ in Arabidopsis plants, respectively, associated with their high level of HT resistance induction. Additionally...