Reduced graphene oxide loaded with tetrahedral framework nucleic acids for combating orthodontically induced root resorption

作者：Wenxiu Yuan, Maotuan Huang, Wenqian Chen, Sihang Chen, Jingwen Cai, Linxin Chen, Hanyu Lin, Kaixun He, Huachen Chen, Wenting Jiang, Yanjing Ou, J Chen · 发表于：Journal of Nanobiotechnology · 年份：2024 · DOI：10.1186/s12951-024-02988-2 · 被引用次数：9 · 研究领域：Advanced biosensing and bioanalysis techniques、Graphene and Nanomaterials Applications、RNA Interference and Gene Delivery

Root resorption occurs outside the root or within the root canal. Regardless of its region, root resorption is irreversible and in severe cases, may even cause tooth loss. Clinically, the external surface root resorption is usually a side effect of orthodontic tooth movement. However, it is frustrating to note that there are almost no effective treatment strategies for orthodontically induced root resorption (OIRR) due to the complexity and ambiguity of etiology. In the current study, we successfully fabricated a delivery complex, reduced graphene oxide nanosheet loading with tetrahedral framework nucleic acids (tFNAs-rGO) through self-assembly. No significant cytotoxicity or organ-toxicity of the tFNAs-rGO complex was observed in cell counting kit-8 assay (CCK-8) and hematoxylin-eosin (HE) staining. Histological staining such as tartrate-resistant acid phosphatase (TRAP) staining and Micro-CT three-dimensional reconstruction were employed to explore the dynamic changes of root and peri-root tissues in OIRR mice. In vitro, we developed an induction microenvironment to testify the effects of the tFNAs-rGO delivery complex on periodontal ligament cells (PDLCs) and macrophages by quantitative RT-PCR, western blot, and immunofluorescence staining. The data showed the reduced the region of root resorption and downregulated osteoclastic activity in OIRR by the tFNAs-rGO complex treatment. Furthermore, our study demonstrated that the tFNAs-rGO delivery complex enhanced osteogenic di...