Ginsenoside Rb1 inhibits cardiomyocyte apoptosis and rescues ischemic myocardium by targeting Caspase-3

作者：Chenhui Zhong, Liyuan Ke, Fen Hu, Zuan Lin, Shuming Ye, Ziyao Zheng, Shengnan Han, Zan Lin, Yuying Zhan, Hu Yan, Peiying Shi, Wen Lei, Hong Yao · 发表于：Journal of Pharmaceutical Analysis · 年份：2024 · DOI：10.1016/j.jpha.2024.101142 · 被引用次数：12 · 研究领域：Ginseng Biological Effects and Applications、Traditional Chinese Medicine Analysis、Autophagy in Disease and Therapy

• Virtual screening was performed to predict the targets of Rb 1 against MI. • Proteomics, CETSA, MD simulation, etc. were used to explore the targets. • Rb 1 does not affect the cleavage of upstream protein Caspase-9 of CASP3. • Rb 1 inhibits CASP3 cleavage by binding to two His121 sites in CASP3. • Rb 1 exerts anti-MI effect via directly targeting CASP3.