Bioactive MgO/MgCO3/Polycaprolactone Multi-gradient Fibers Facilitate Peripheral Nerve Regeneration by Regulating Schwann Cell Function and Activating Wingless/Integrase-1 Signaling

作者：Zhi Yao, Ziyu Chen, Xuan He, Yihao Wei, Junyu Qian, Qiang Zong, Shuxian He, Lili Song, Lijia Ma, Sien Lin, Linlong Li, Lixiang Xue, Siu Ngor Fu, Jin Zhang, Ye Li, Deli Wang · 发表于：Advanced Fiber Materials · 年份：2024 · DOI：10.1007/s42765-024-00489-3 · 被引用次数：24 · 研究领域：Nerve injury and regeneration、Bone Tissue Engineering Materials、Graphene and Nanomaterials Applications

Abstract Peripheral nerve defects present complex orthopedic challenges with limited efficacy of clinical interventions. The inadequate proliferation and dysfunction of Schwann cells within the nerve scaffold impede the effectiveness of nerve repair. Our previous studies suggested the effectiveness of a magnesium-encapsulated bioactive hydrogel in repairing nerve defects. However, its rapid release of magnesium ions limited its efficacy to long-term nerve regeneration, and its molecular mechanism remains unclear. This study utilized electrospinning technology to fabricate a MgO/MgCO 3 /polycaprolactone (PCL) multi-gradient nanofiber membrane for peripheral nerve regeneration. Our findings indicated that by carefully adjusting the concentration or proportion of rapidly degradable MgO and slowly degradable MgCO 3 , as well as the number of electrospun layers, the multi-gradient scaffold effectively sustained the release of Mg 2+ over a period of 6 weeks. Additionally, this study provided insight into the mechanism of Mg 2+ -induced nerve regeneration and confirmed that Mg 2+ effectively promoted Schwann cell proliferation, migration, and transition to a repair phenotype. By employing transcriptome sequencing technology, the study identified the Wingless/integrase-1 (Wnt) signaling pathway as a crucial mechanism influencing Schwann cell function during nerve regeneration. After implantation in 10 mm critically sized nerve defects in rats, the MgO/MgCO 3 /PCL multi-gradient nanof...