Banxia-Yiyiren alleviates insomnia and anxiety by regulating the gut microbiota and metabolites of PCPA-induced insomnia model rats

作者：Liang Wang, Xiaorong Qi, Shuo Wang, Chujiao Tian, Tao Zou, Zihan Liu, Qi Chen, Yingfan Chen, Yunshan Zhao, Shaodan Li, Minghui Yang, Ningli Chai · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1405566 · 被引用次数：9 · 研究领域：Gut microbiota and health、Metabolomics and Mass Spectrometry Studies、Sleep and related disorders

Objective This study aims to clearly define the effects of Banxia-Yiyiren on the gut microbiota and its metabolites in a para-chlorophenylalanine-induced insomnia model and the possible underlying mechanisms involved. Materials and methods We employed 16S ribosomal ribonucleic acid (rRNA) gene sequencing combined with metabonomic analysis to explore the mutual effects of the PCPA-induced insomnia model and the gut microbiota and the intrinsic regulatory mechanism of Banxia-Yiyiren on the gut microbiota and metabolites in the PCPA-induced insomnia model. Results Banxia-Yiyiren was identified by mass spectrometry to include amino acids, small peptides, nucleotides, organic acids, flavonoids, fatty acids, lipids, and other main compound components. The elevated plus maze (EPM) test results revealed that high-dose Banxia-Yiyiren may increase willingness to explore by improving anxiety-like symptoms caused by insomnia. Through 16S rRNA gene sequencing, at the phylum level, compared with those in G1, the relative abundances of Bacteroidota and Proteobacteria in G2 increased, whereas the relative abundance of Firmicutes decreased. At the genus level, compared with those in G1, the relative abundances of Prevotella_9 , Prevotella , Ralstonia , Escherichia-Shigella , and UCG-005 in G2 increased, whereas the relative abundances of Lactobacillus , Ligilactobacillus , Alloprevotella , Blautia , and Prevotellaceae_NK3B31_group decreased. The metabolomics analysis results revealed 1,574 me...