Metabolomic and transcriptomic analyses reveal MYB-Related genes involved in drought resistance in grafted potatoes via the flavonoid pathway

作者：Yinqiao Jian, Chunyan Gao, Yangyang Shang, Junhong Qin, Shaoguang Duan, Chunsong Bian, Guangcun Li · 发表于：Plant Stress · 年份：2024 · DOI：10.1016/j.stress.2024.100665 · 被引用次数：10 · 研究领域：Plant-Microbe Interactions and Immunity、Plant Disease Management Techniques、Plant tissue culture and regeneration

• Grafting with drought-tolerant rootstocks enhances scion drought resistance in potatoes. • Flavonoids are significantly enriched in leaves and roots, linked to drought tolerance. • MYB or MYB-related hub genes are identified as key regulators of drought resistance. • Metabolomic and transcriptomic analyses reveal mechanisms of grafting-mediated drought resistance. • The MYB-WD40-bHLH complex plays a crucial role in the drought stress response. Drought poses a significant challenge to global potato production. Grafting, a classical horticultural technique, has the potential to enhance resistance to both biotic and abiotic stresses. However, the use of grafting to improve drought resistance in potatoes, along with the underlying genetic and regulatory changes remains inadequately documented. In this study, we investigated the drought phenotypes, as well as the metabolomic and transcriptomic profiles of leaves and roots in self-grafted (drought-sensitive scion/ drought-sensitive rootstock, SS) and hetero-grafted (drought-sensitive scion/drought-tolerant rootstock, ST) potatoes. After 40 days, hetero-grafts exhibited greater drought resistance as well as lower dry matter content and higher soluble sugar content compared to self-grafts, indicating that grafting with drought-tolerant rootstocks can enhance the drought resistance of the scion and revealed physiological process. Metabolomic analysis revealed a significant enrichment of flavonoids, particularly in comparisons betwee...