Enhanced Bacterial and Biofilm Adhesion Resistance of ALD Nano-TiO2 Coatings Compared to AO Coatings on Titanium Abutments

作者：Yu Pan, Lili Cao, Libing Chen, Linjuan Gao, Xia Wei, Honglei Lin, Lei Jiang, Yinghui Wang, Hui Cheng · 发表于：International Journal of Nanomedicine · 年份：2024 · DOI：10.2147/ijn.s482478 · 被引用次数：11 · 研究领域：Bone Tissue Engineering Materials、Titanium Alloys Microstructure and Properties、Dental Implant Techniques and Outcomes

Purpose: The study was intended to compare the surface properties and the bacterial and biofilm adhesion resistance of two potential antibacterial nanometer titanium dioxide (nano-TiO 2 ) coatings on dental titanium (Ti) abutments prepared by atomic layer deposition (ALD) and the anodic oxidation (AO) techniques. Methods: Nano-TiO₂ coatings were developed using ALD and AO techniques and applied to Ti surfaces. The surface properties and the bacterial and biofilm adhesion resistance of these coatings were evaluated against commonly used Ti and Zirconia (ZrO₂) surfaces. The chemical compositions, crystalline forms, surface topography, roughness and hydrophilicity were characterized. The antibacterial performance was assessed by the scanning electron microscope (SEM), the Colony-forming unit (CFU) assay and the 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay using in vitro models of Staphylococcus aureus ( S. aureus ), Streptococcus mutans ( S. mutans ), and Porphyromonas gingivalis ( P. gingivalis ) in both single- and mixed-species bacterial compositions. Results: ALD-prepared nano-TiO₂ coatings resulted in a dense, smooth, and less hydrophilic surface with an anatase phase, significantly reducing the adhesion of the three bacteria by over 50%, comparable to ZrO₂. In contrast, AO-prepared coatings led to a less hydrophilic surface, characterized by various nano-sized pores within the oxide film. This alteration, however, had no impact on the adhesion ...