Menstrual blood‐derived mesenchymal stem cells combining with platelet‐rich plasma infusion in endometrium repair

作者：Yang Wang, Wenxin Liu, Hui Xu, Liyuan Dong, Yalou Jiao, Zhe Qiao · 发表于：Journal of obstetrics and gynaecology research · 年份：2024 · DOI：10.1111/jog.16135 · 被引用次数：4 · 研究领域：Gynecological conditions and treatments、Reproductive System and Pregnancy、Endometriosis Research and Treatment

OBJECTIVE: Thin endometrium caused by various factors affects the conception rate of females worldwide; however, current medications are still insufficient. Therefore, a novel approach is needed. We previously reported the effect of menstrual blood-derived mesenchymal stem cells (MenSCs) in ameliorating ethanol-induced endometrial injuries. In the present study, we aimed to investigate whether the effect of MenSCs is enhanced by a combination of platelet-rich plasma (PRP) infusion. METHODS: An endometrial injury mouse model was established by infiltrating 95% ethanol for 15 s into the uterus, followed by MenSCs, PRP, or MenSCs + PRP treatment. Pathological changes were observed by HE staining. The expression of CK18, vimentin, ItgαVβ3, and VEGF was determined using IHC staining and WB blotting. RESULTS: Compared with the model, MenSCs, PRP, and MenSCs + PRP treatments significantly improved endometrial damage and thickness, with the combined therapy displaying the most pronounced efficacy. The density of CK18-, vimentin-, and ItgαVβ3 positive cells increased most significantly in the MenSCs + PRP group of mice. In addition, the protein expression of CK18, vimentin, and VEGF was significantly upregulated after MenSCs + PRP, MenSCs, and PRP treatment, with MenSCs + PRP therapy showing the best efficacy. CONCLUSION: MenSCs + PRP therapy is more beneficial for ameliorating ethanol-induced endometrial damage than MenSCs or PRP alone, providing a basis for the investigation of nove...