Investigating T Cell Immune Dynamics and IL-6’s Duality in a Microfluidic Lung Tumor Model

作者：Parvaneh Sardarabadi, Kang‐Yun Lee, Wei-Lun Sun, Amir Asri Kojabad, Cheng‐Hsien Liu · 发表于：ACS Applied Materials & Interfaces · 年份：2024 · DOI：10.1021/acsami.4c09065 · 被引用次数：11 · 研究领域：3D Printing in Biomedical Research、Cancer Cells and Metastasis、Immune cells in cancer

High Resolution Image Download MS PowerPoint Slide Interleukin 6 (IL-6), produced by immune cells, is crucial in promoting T cell trafficking to infection and inflammation sites, influencing various physiological and pathological processes. Concentrations of IL-6 and other cytokines and chemokines can influence T cell differentiation and activation. Understanding the dual faces of IL-6 within the tumor microenvironment is crucial to understanding its role. A flow-based microsystem was designed to investigate CD4 + T cell activation in response to different IL-6 gradients in an under-control 3D culture. The study found that cancer cells’ response to varying IL-6 concentrations was dynamic and dose-sensitive, with immune cell migration rates showing sensitivity to the IL-6 gradient. A549 cell expansion increases gradually and time-dependently with 50 ng of IL-6, while Jurkat cell migration follows a time-dependent pattern. However, when a total of 100 ng IL-6 concentration is applied, A549 cells expand rapidly, potentially influencing Jurkat cell migration. Jurkat cell mobility is lower, possibly due to increased A549 cell presence and heightened cell–cell interactions. Different IL-6 concentration gradients can modulate the expression of some CD markers like CD69 and programed cell death protein 1 in CD4 + T cells, suggesting that IL-6 concentration gradients affect immune cell phenotypes. This suggests that IL-6 plays a crucial role in activating T helper cells and may be inv...