Developing super rice varieties resistant to rice blast with enhanced yield and improved quality

作者：Naihui Guo, Ruihu An, Zongliang Ren, Jun Jiang, Bonian Cai, Shikai Hu, Gaoneng Shao, Guiai Jiao, Lihong Xie, Ling Wang, Fengli Zhao, Shaoqing Tang, Zhonghua Sheng, Peisong Hu · 发表于：Plant Biotechnology Journal · 年份：2024 · DOI：10.1111/pbi.14492 · 被引用次数：13 · 研究领域：Rice Cultivation and Yield Improvement、GABA and Rice Research、Genetic Mapping and Diversity in Plants and Animals

Rice blast, caused by the Magnaporthe oryzae, is the most detrimental disease to rice. Yield losses caused by this disease were from 10% to 30% in rice planting areas (Skamnioti and Gurr, 2009); severe cases may even lead to complete cessation of production (Parker et al., 2008). Cloning rice blast resistant genes and applying them to cultivate resistant varieties is a practical and effective method for controlling rice blast disease. So far, more than 70 disease resistance genes and QTLs have been identified, of which at least 25 have been cloned and used in disease resistance breeding (Luo et al., 2017). The Pi2 gene encodes a protein with a nucleotide-binding site and leucine-rich repeat (LRR) domain (Zhou et al., 2006), and is one of the most broad-spectrum and efficient resistance genes to rice blast, exhibiting resistance to 36 out of 43 rice blast strains from 13 countries (Liu et al., 2002). However, many elite varieties do not carry Pi2 gene, which seriously hinders the widespread and sustainable promotion of these varieties. Marker-assisted selection (MAS) is one of the important technologies in modern breeding. It uses molecular markers to quickly detect individual plants carrying target genes, greatly improving breeding efficiency and saving breeding costs. Previously, Jiang et al. (2015) developed blast-resistant thermosensitive genic male sterile (TGMS) lines by employing MAS. High-density whole genome single nucleotide polymorphism (SNP) chips are also a techno...