Insights into chemoautotrophic traits of a prevalent bacterial phylum CSP1-3, herein Sysuimicrobiota

作者：Lan Liu, Zheng‐Han Lian, LV Ai-ping, Nimaichand Salam, Jianchao Zhang, Mengmeng Li, Weimin Sun, Sha Tan, Zhen‐Hao Luo, Lei Gao, Yuan Yang, Yu-Zhen Ming, Yu‐Ting OuYang, Yu-Xian Li, Ze‐Tao Liu, Chao‐Jian Hu, Ying Chen, Zheng‐Shuang Hua, Wensheng Shu, Brian P. Hedlund, Wen-Jun Li, Jian‐Yu Jiao · 发表于：National Science Review · 年份：2024 · DOI：10.1093/nsr/nwae378 · 被引用次数：9 · 研究领域：Gut microbiota and health、Clostridium difficile and Clostridium perfringens research、Oral microbiology and periodontitis research

ABSTRACT Candidate bacterial phylum CSP1-3 has not been cultivated and is poorly understood. Here, we analyzed 112 CSP1-3 metagenome-assembled genomes and showed they are likely facultative anaerobes, with 3 of 5 families encoding autotrophy through the reductive glycine pathway (RGP), Wood–Ljungdahl pathway (WLP) or Calvin-Benson-Bassham (CBB), with hydrogen or sulfide as electron donors. Chemoautotrophic enrichments from hot spring sediments and fluorescence in situ hybridization revealed enrichment of six CSP1-3 genera, and both transcribed genes and DNA-stable isotope probing were consistent with proposed chemoautotrophic metabolisms. Ancestral state reconstructions showed that the ancestors of phylum CSP1-3 may have been acetogens that were autotrophic via the RGP, whereas the WLP and CBB were acquired by horizontal gene transfer. Our results reveal that CSP1-3 is a widely distributed phylum with the potential to contribute to the cycling of carbon, sulfur and nitrogen. The name Sysuimicrobiota phy. nov. is proposed.