S2053 Targeted Breath Biopsy Profiling of Induced Biomarkers Unveils a Metabolic Adaptation in Cirrhosis Toward Alcohol Production

作者：Giuseppe Ferrandino, Federico Ricciardi, Antonio Murgia, Menisha Manhota, Daniela Fonseca, Louise Nicholson-Scott, Chloe Fitzpatrick, Olga Gandelman, Max Allsworth, Billy Boyle, Agnieszka Smolinska, Huw Davies, Alexandra Ginesta Frings, Jorge Contreras, Victor Gabrielli, Claudia Asenjo‐Lobos, Francisca Bascur, Viviana Barrientos, Nataly Clavo, Melissa Jerez, Luis Méndez · 发表于：The American Journal of Gastroenterology · 年份：2024 · DOI：10.14309/01.ajg.0001037580.02027.fb · 被引用次数：1 · 研究领域：Advanced Chemical Sensor Technologies、Alcohol Consumption and Health Effects、Metabolomics and Mass Spectrometry Studies

Introduction: Subjects with chronic liver disease showed higher circulating ethanol levels after fasting or carbohydrates ingestion. However, clinical functional data about the capability of the injured liver to cope with alcohols remain limited. In this study we used exhaled breath analysis to explore the conversion of an alcohol to the corresponding ketone, and a ketone to the corresponding alcohol, to establish the suitability of potential metabolic alterations and identify subjects with cirrhosis using a non-invasive breath test Methods: We selected 2-butanol, and 2-pentanone (the substrates) as an alcohol and a ketone based on their safe human consumption, reported hepatic metabolism, and presence in breath. Hepatic metabolism was confirmed by treating human suspension hepatocytes with 10 ng/µl of 2-butanol, and 2-pentanone to measure their conversion to respectively 2-butanone, and 2-pentanol (the bioproducts) by using headspace analysis coupled with gas-chromatography mass-spectrometry (GC-MS). Clinical metabolism was evaluated by analysing the breath profile of 14 subjects with cirrhosis and 15 controls before and at different timepoints (10 - 120 minutes) after ingestion of 2-butanol (100 mg) and 2-pentanone (100 mg), using Breath Biopsy OMNI Results: Bioproducts were present in primary hepatocytes treated with the substrates and absent in control conditions omitting primary hepatocytes or substrates. After an overnight fasting all the participants showed breath leve...