Shisandra Decoction Alleviates Parkinson’s Disease Symptoms in a Mouse Model Through PI3K/AKT/mTOR Signalling Pathway

作者：Yawen Pan, Mojinzi Chen, Lulu Pan, Qiuling Tong, Zhiqing Cheng, Sujin Lin, Rongrong Pan, Mengyuan Chen, Yinghao Zhi · 发表于：Neuropsychiatric Disease and Treatment · 年份：2024 · DOI：10.2147/ndt.s476969 · 被引用次数：9 · 研究领域：Parkinson's Disease Mechanisms and Treatments、Plant-derived Lignans Synthesis and Bioactivity、Autophagy in Disease and Therapy

Purpose: The present study aimed to characterize neuroprotective effects of Schisandra Decoction (Sch D) treatment in a mouse model of Parkinson's disease (PD), and to explore underlying mechanisms focused on the mammalian target of rapamycin (mTOR) signaling pathway. Materials and Methods: 50 male C57 BL/6 mice were randomly assigned to either control (n = 10) or 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model (n = 40) groups. PD mice were further divided into four groups of ten mice each: MPTP group, LY294002 group, Sch D group, and LY2940002 + Sch D group. Mice from each group were assessed in pole climbing, rotary rod and open field tests. Brain Tyrosine hydroxylase (TH) protein was observed using immunohistochemistry. mRNA levels of PTEN, PI3K and LC3 in brain tissue were measured using RT-PCR. Protein levels of PTEN, PI3K, Akt, p-Akt, mTOR, p-mTOR, p70s6K, p62, LC3II / I, α-synuclein (α-syn), TH in brain tissue were assessed by Western blotting (WB). Results: In behavioral tests, PD mice treated with Sch D showed reduced pole climbing time, longer rotarod duration, and greater distance traveled. In terms of neuroprotection, PD mice in the Sch D group exhibited higher levels of TH protein and enhanced α-syn clearance. Regarding autophagy, compared to the control group, mice in the MPTP group had elevated PTEN protein expression, which inhibited PI3K, p-AKT/AKT, and p-mTOR/mTOR protein levels, decreased LC3II/I protein expression, and increased P62 protein expre...