The Prognostic Significance of CTSV Expression in Patients with Hepatocellular Carcinoma

作者：Yu‐Ping He, Minghao Xu, Chenhao Zhou, Qiongzhu Dong, Zhiqiu Hu, Ning Ren · 发表于：International Journal of General Medicine · 年份：2024 · DOI：10.2147/ijgm.s467179 · 被引用次数：5 · 研究领域：Protease and Inhibitor Mechanisms、Ferroptosis and cancer prognosis、Oral microbiology and periodontitis research

Purpose: Cathepsin V (CTSV) is a cysteine protease peptidase, which is typically upregulated in cancer and is associated with various oncogenic processes, such as angiogenesis, proliferation, apoptosis, and invasion. The study explored the role of CTSV in hepatocellular carcinoma (HCC) and its potential as a potential biomarker. Patients and Methods: This study collected tumor and peritumoral archived specimens from 180 HCC patients who underwent surgical resection at Zhongshan Hospital, Fudan University (Shanghai, China) between 2009 and 2010. We extracted data from the TCGA and GEO databases and conducted differential expression analysis, univariate Cox regression, and Kaplan-Meier analysis. Ultimately, we determined that CTSV may emerge as a potential biomarker. Then, immunohistochemical staining for CTSV was performed on tumors and adjacent tissues of HCC patients, and a Cox proportional hazards model was constructed to evaluate the prognostic significance of CTSV expression levels. Applied functional enrichment analysis to reveal the underlying molecular mechanisms. Utilized ssGSEA enrichment analysis and TIMER2.0 algorithm to explore the correlation between CTSV expression and immune cells in HCC. In vitro and in vivo experiments were conducted using human liver cancer cell lines to further validate the clinical application value of CTSV. Results: In this study, we observed that CTSV expression was notably elevated in HCC (P < 0.001), and identified a significant associ...