A small-molecule carrier for the intracellular delivery of a membrane-impermeable protein with retained bioactivity

作者：Xiqi Ma, Zhixiong Zhang, Andrea Barba‐Bon, Dongxue Han, Zichun Qi, Baosheng Ge, Hua He, Fang Huang, Werner M. Nau, Xiaojuan Wang · 发表于：Proceedings of the National Academy of Sciences · 年份：2024 · DOI：10.1073/pnas.2407515121 · 被引用次数：27 · 研究领域：Boron Compounds in Chemistry、Supramolecular Chemistry and Complexes、Advanced biosensing and bioanalysis techniques

Intracellular protein delivery has the potential to revolutionize cell-biological research and medicinal therapy, with broad applications in bioimaging, disease treatment, and genome editing. Herein, we demonstrate successful delivery of a functional protein, cytochrome c (CYC), by using a boron cluster anion as molecular carrier of the superchaotropic anion type (B 12 Br 11 OPr 2– ). CYC was delivered into lipid bilayer vesicles as well as living cells, with a cellular uptake ratio approaching 90%. Mechanistic studies showed that CYC was internalized into cells through a permeation pathway directly into the cytoplasm, bypassing endosomal entrapment. Upon carrier-assisted internalization, CYC retained its bioactivity, as reflected by an induced cell apoptosis rate of 25% at low dose (1 µM). This study furbishes a direct protein delivery method by a molecular carrier with high efficiency, confirming the potential of inorganic cluster ions as protein transport vehicles with an extensive range of future cell-biological or biomedical applications.