Existence and distribution of the microbiome in tumour tissues of children with hepatoblastoma

作者：Jinghua Cui, Xiaoran Li, Qun Zhang, Bing Du, Zanbo Ding, Chao Yan, Guanhua Xue, Lin Gan, Junxia Feng, Zheng Fan, Ziying Xu, Zihui Yu, Tongtong Fu, Yanling Feng, Hanqing Zhao, Yiming Kong, Xiaohu Cui, Ziyan Tian, Quanda Liu, Jing Yuan · 发表于：Heliyon · 年份：2024 · DOI：10.1016/j.heliyon.2024.e39547 · 被引用次数：4 · 研究领域：Gut microbiota and health、Digestive system and related health、Diet and metabolism studies

Cancer microbiota have recently been demonstrated in several cancer types. The microbiome enhances inflammation in the cancer microenvironment and affects the disease pathology by regulating tumourigenesis, cancer progression, and chemotherapy resistance. Hepatoblastoma (HB), the most common childhood malignant tumour, is a malignant embryonic tumour. However, the pathogenesis and molecular basis of HB remain poorly understood. In this study, to explore the existence and distribution of the microbiome in tumour tissues and adjacent non-tumour tissues of children with HB, we mainly performed 16S rDNA sequencing, and the results showed that the diversity and abundance of the microbiome in children with HB were significantly different between HB tumours and adjacent non-tumour tissues ( p < 0.01). At the phylum level, the dominant microbiome in the tumour tissues were Proteobacteria, Bacteroidetes, and Firmicutes. At the genus level, Ruminococcus was more abundant in HB tumours than in the adjacent non-tumour tissues. Simultaneously, the abundances of Bacteroides , Parabacteroides , Lachnospiracea -NK4A136, and Alistipes in HB tumours were lower than those in the adjacent non-tumour tissues. In addition, Romboutsia strongly correlated with alpha-fetoprotein, an important indicator of HB. Sphingomonas was abundant in primary HB tumours, whereas Oscillibacter and Pandoraea were abundant in metastatic HB tumours. However, whether these bacteria are associated with HB needs further ...