CRISPR/Cas12a assay for amol level microRNA by combining enzyme-free amplification and single particle analysis

作者：Chengchao Zhang, Xin Zhao, Xiao Chen, Lin Xu, Zili Huang, Jianyu Hu, Rui Liu, Yi Lv · 发表于：Chemical Communications · 年份：2024 · DOI：10.1039/d4cc04534c · 被引用次数：12 · 研究领域：CRISPR and Genetic Engineering、Advanced biosensing and bioanalysis techniques、RNA Interference and Gene Delivery

CRISPR/Cas systems are increasingly utilized for sensitive miRNA detection through enzyme-based pre-amplification. To address challenges such as high costs, non-specific amplification, and interference from primer residues in pre-amplification strategies, herein a dual amplification CRISPR miRNA assay was developed by combining enzyme-free HCR with single-particle analysis. Attomolar detection limits, excellent selectivity, and practicability were achieved by applying this method.