Limosilactobacillus-related 3-OMDP as a potential therapeutic target for depression

作者：Qi Zhong, Wentao Wu, Jing Xie, Jiaolin Wang, Ke Xu, Yi Ren, Jianjun Chen, Peng Xie · 发表于：Annals of Medicine · 年份：2024 · DOI：10.1080/07853890.2024.2417179 · 被引用次数：6 · 研究领域：Tryptophan and brain disorders、Gut microbiota and health、Stress Responses and Cortisol

OBJECTIVE: Gut microbiota was closely involved in the pathogenesis of depression, but the underlying molecular mechanisms in depression remained unclear. This study was conducted to investigate the relationship between neurotransmitters/inflammatory factors and gut microbiota in depressed mice. MATERIALS AND METHODS: A chronic social defeat stress (CSDS) depression model was established. Gut microbial composition was detected in faeces, neurotransmitters were detected in faeces, colon, blood and hippocampus, and inflammatory factors were detected in hippocampus. After a key neurotransmitter was identified, intervention experiment was conducted to explore whether it could improve depressive-like behaviours. RESULTS: Six differential genera in faeces, 14 differential neurotransmitters in gut-brain axis, and two differential inflammatory factors (interleukin-1 beta (IL-1β) and interleukin-6 (IL-6)) in hippocampus were identified in depressed mice. There were significant correlations among differential genera, differential neurotransmitters and IL-1β/IL-6. Among these differential neurotransmitters, 3-O-Methyldopa (3-OMDP) was found to be consistently decreased in faeces, colon, blood and hippocampus, and 3-OMDP was significantly correlated to Limosilactobacillus and IL-1β. After receiving 3-OMDP, the depressive-like behaviours in depressed mice were improved and the increased IL-1β/IL-6 levels were reversed. CONCLUSIONS: These results indicated that gut microbiota might affect h...