Seasonal dynamics of bacterial composition and functions in biological treatment of coking wastewater

作者：Zhi-Jie Tan, Wenli Chen, Ziyu Guo, Xing-Yuan Xu, Junting Xie, Jiangpeng Dai, Yuexia Lin, Binbin Sheng, Sergei Preis, Chaohai Wei, Shuang Zhu · 发表于：Applied Microbiology and Biotechnology · 年份：2024 · DOI：10.1007/s00253-024-13274-4 · 被引用次数：12 · 研究领域：Microbial Community Ecology and Physiology、Anaerobic Digestion and Biogas Production、Microbial Fuel Cells and Bioremediation

Seasonal dynamics of bacterial composition and functions were demonstrated for the biological fluidized-bed bioreactors combined in the anoxic/aerobic1/aerobic2 (AOO) coking wastewater (CWW) treatment sequences. The bacterial composition and functions in the CWW activated sludge samples were revealed by 16S rRNA genes amplicon sequencing. Thiobacillus, Cloacibacterium, Alkaliphilus and Pseudomonas were determined as core genera with seasonal changes. Mutable microbial community composition fluctuated in different seasons in same bioreactor. Distributions of predicted KEGG pathways along four seasons consistently demonstrated enrichment in biodegradation of carbon- and nitrogen-containing compounds. The major contaminants were removed from CWW by biochemical pathway of xenobiotics biodegradation and metabolism. This Level 2 pathway mainly owned the Level 3 pathways of benzoate degradation, drug metabolism-other enzymes, drug metabolism-cytochrome P450, metabolism of xenobiotics by cytochrome P450, and aminobenzoate degradation. The RDA results showed that dissolved oxygen with seasonal fluctuation was the main parameter shaping the microbial community. The observed dynamics within the microbial community composition, coupled with the maintained stability of CWW treatment efficiencies and a consistent profile of microbial functional pathways, underscore the presence of functional redundancy in the AOO bioreactors. The study underscored stable and effective operational performan...