CNGC20 plays dual roles in regulating plant growth and immunity in Brassica napus

作者：Yingying Tan, Guowei Huang, Haiyan Fan, Tao Wu, Zhilin Guan, Kede Liu · 发表于：The Crop Journal · 年份：2024 · DOI：10.1016/j.cj.2024.09.012 · 被引用次数：3 · 研究领域：Plant-Microbe Interactions and Immunity、Plant Molecular Biology Research、Plant tissue culture and regeneration

Inflorescence architecture is determined by inflorescence length, branch angles and the density of siliques, which affects planting density, lodging resistance and mechanical operation in rapeseed. However, the molecular mechanisms controlling inflorescence architecture are poorly understood, restricting the progress of breeding varieties with ideal plant architecture in oilseed rape. In this study, we have identified and characterized a rapeseed inflorescence development mutant, reduced inflorescence length ( ril ), which exhibits determinate and shortened inflorescences, reduced plant height, compact branches, and increased silique density. Through BSA-seq and map-based cloning, we find that RIL encodes a cyclic nucleotide-gated channel 20 ( BnaA01.CNGC20 ). A substitution of proline at the 304th position to leucine (P304L) was identified in the conserved transmembrane domain of BnaA01.CNGC20. This P304L substitution neither affects the subcellular localization and self-assembly of BnaA01.CNGC20, nor disrupts the interactions with BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1), which interacts with CNGC20 and phosphorylates it to regulate Ca 2+ channel stability. However, the P304L substitution increases channel activity and Ca 2+ influx, which in turn induces immune responses such as cell death, H 2 O 2 accumulation, upregulation of pathogenesis-related genes, and pattern-triggered immunity. The enhanced immunity improves the resistance to Leptosphaeria ...