A multi-omics insight on the interplay between iron deficiency and N forms in tomato

作者：Arianna Lodovici, Sara Buoso, Begoña Miras‐Moreno, Luigi Lucini, Nicola Tomasi, Pascual García-Pérez, Roberto Pinton, Laura Zanin · 发表于：Frontiers in Plant Science · 年份：2024 · DOI：10.3389/fpls.2024.1408141 · 被引用次数：4 · 研究领域：Plant Micronutrient Interactions and Effects、Plant nutrient uptake and metabolism、Plant Stress Responses and Tolerance

Introduction: Nitrogen (N) and iron (Fe) are involved in several biochemical processes in living organisms, and their limited bioavailability is a strong constraint for plant growth and yield. This work investigated the interplay between Fe and N nutritional pathways in tomato plants kept under N and Fe deficiency and then resupplied with Fe and N (as nitrate, ammonium, or urea) through a physiological, metabolomics and gene expression study. Results: After 24 hours of Fe resupply, the Fe concentration in Fe-deficient roots was dependent on the applied N form (following the pattern: nitrate > urea > ammonium > Fe-deficient control), and whereas in leaves of urea treated plants the Fe concentration was lower in comparison to the other N forms. Untargeted metabolomics pointed out distinctive modulations of plant metabolism in a treatment-dependent manner. Overall, N-containing metabolites were affected by the treatments in both leaves and roots, while N form significantly shaped the phytohormone profile. Moreover, the simultaneous application of Fe with N to Fe-deficient plants elicited secondary metabolites' accumulation, such as phenylpropanoids, depending on the applied N form (mainly by urea, followed by nitrate and ammonium). After 4 hours of treatment, ammonium- and urea-treated roots showed a reduction of enzymatic activity of Fe(III)-chelate reductase (FCR), compared to nitrate or N-depleted plants (maintained in Fe deficiency, where FCR was maintained at high levels). ...