Importance of Ultraviolet-C (UV-C) Emitter Configuration for the Attenuation of Staphylococcus aureus and Candida auris Pathogens

作者：Randy W. Loftus, Carmen T Brindeiro, Franklin Dexter, Michelle C. Parra, Soyun M Hwang, Brendan T. Wanta, Debra J. Szeluga, Brent Hadder, Melinda Seering, Jonathan E. Charnin · 发表于：Cureus · 年份：2024 · DOI：10.7759/cureus.71612 · 被引用次数：3 · 研究领域：Listeria monocytogenes in Food Safety、Bacterial Identification and Susceptibility Testing、Vibrio bacteria research studies

Background: The relative importance of different ultraviolet-C (UV-C) emitter configurations on the attenuation of vegetative bacterial and fungal pathogens has not been assessed. We hypothesized that emitter configuration would impact the efficacy of UV-C attenuation of Staphylococcus aureus (S. aureus) and Candida auris (C. auris) pathogens. Methods: American Type Culture Collection (ATCC) S. aureus (ATCC 6538) and C. auris (ATCC MYA-5001) carriers (ReadyNowTM Test Carriers, Stratix Labs Corporation, Saint Paul, MN) were mounted on an aluminum stand along with three calibrated radiometers (International Light Technologies model ILT1270, Peabody, MA). Five UV-C emitter configurations were assessed, including three emitters with a triangular configuration about the stand and each rotating 360° (1), one emitter facing the stand and rotating 360° (2), three emitters facing the stand in a linear configuration and each rotating 5° (3), one emitter facing the stand and rotating 5° (4), and one emitter facing the stand and rotating 90° (5). Three serial experiments were conducted. The first experiment involved the establishment of the minimally effective irradiation dose (mean and standard deviation mJ/cm2) required to achieve no growth (6-log reduction (LR)) with direct exposure to pathogen carriers positioned at the center of the lamp. We then assessed the relative efficacy of delivery of the minimally effective dose via the five emitter configurations in attenuating polycarbonat...